Cloning, Expression, and Characterization of a Human Inosine Triphosphate Pyrophosphatase Encoded by the ITPAGene
نویسندگان
چکیده
منابع مشابه
Cloning, expression, and characterization of a human inosine triphosphate pyrophosphatase encoded by the itpa gene.
ITP and dITP exist in all cells. dITP is potentially mutagenic, and the levels of these nucleotides are controlled by inosine triphosphate pyrophosphatase (EC ). Here we report the cloning, expression, and characterization of a 21.5-kDa human inosine triphosphate pyrophosphatase (hITPase), an enzyme whose activity has been reported in many animal tissues and studied in populations but whose pro...
متن کاملP-121: Cloning and Expression of The Inosine Triphosphate Pyrophosphatase Gene Variant II in E.coli
Background Environmental and cellular inappropriate conditions can cause damages to cells nucleotide poll. Deamination and oxidation damages interfere with cell�s vital reactions. Inosine triphosphate pyrophosphatase (ITPA), an evolutionary conserved enzyme, plays a critical role in elimination of non-canonical bases. In human genome, the ITPA gene is located on chromosome 20 short arm and tran...
متن کاملStructure of the orthorhombic form of human inosine triphosphate pyrophosphatase.
The structure of human inosine triphosphate pyrophosphohydrolase (ITPA) has been determined using diffraction data to 1.6 A resolution. ITPA contributes to the accurate replication of DNA by cleansing cellular dNTP pools of mutagenic nucleotide purine analogs such as dITP or dXTP. A similar high-resolution unpublished structure has been deposited in the Protein Data Bank from a monoclinic and p...
متن کاملcomparison of inosine triphosphate pyrophosphatase gene expression in chronic myelogenous leukemia patients and controls
objective: one of the most significant damages to the genetic material is oxidative deamination of dna and free nucleotides in the cell pool. incorporation of deaminated purine nucleotides such as inosine triphosphate (itp, ditp) into the dna can increase the frequency of base substitution mutation. it has been suggested that presence and accumulation of these rough nucleotides can lead to gene...
متن کاملITPA (inosine triphosphate pyrophosphatase): from surveillance of nucleotide pools to human disease and pharmacogenetics.
Cellular nucleotide pools are often contaminated by base analog nucleotides which interfere with a plethora of biological reactions, from DNA and RNA synthesis to cellular signaling. An evolutionarily conserved inosine triphosphate pyrophosphatase (ITPA) removes the non-canonical purine (d)NTPs inosine triphosphate and xanthosine triphosphate by hydrolyzing them into their monophosphate form an...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2001
ISSN: 0021-9258
DOI: 10.1074/jbc.m011084200